Promoter is an essential element for regulating the effective transcription of genes and an important part of constructing gene expression vectors. It plays an important role in studying the mechanism of biological development, improving the expression yield of target genes and conducting gene therapy. effect.
Isolating different types of gene promoters has become one of the important tasks of genetic engineering, and the promoter probe (promoter: probe) vector is an effective, economical, and rapid tool for isolating gene promoters. In addition to the necessary components of a plasmid vector, the promoter probe vector must also contain detection components.
The detection component consists of two parts, namely a genetic marker gene that has lost its promoter and is easy to detect, and a cloning site. Currently, the main genes used to detect genetic markers include antibiotic resistance genes and green fluorescent protein genes emphasis: role=italicgfemphasisp, etc.