1. Mycoplasma culture:
a. Collect specimens, usually urogenital swabs or swabs, rarely prostatic fluid, semen, synovial fluid, or fallopian tube, rectal biopsy In recent years, primary urine sample centrifuge has been used to replace urethral swabs. When taking a swab, insert the swab 2-4cm into the male urethra and wipe it hard. This method can easily cause urethral damage and secondary infection. Use with caution. Women need to clean the junction of cervical squamous and columnar epithelium first, and use a cell brush to collect cervical specimens, which can increase the number of infected cells and is more sensitive than cotton swabs.
b. Commonly used culture media are beef heart infusion or peptone, and contain 1% fresh yeast infusion, 10-20% animal serum and 0.5% sodium chloride. Glucose and arginine can also be added. To promote the growth of MH and MG, add urea for UU metabolism, and add an appropriate amount of penicillin to inhibit miscellaneous bacteria.
2. Serological identification method: The most commonly used is the agar diffusion method, which is to inoculate mycoplasma onto an agar dish. Then put the filter paper soaked in an appropriate amount of serum on the agar surface to observe which one can inhibit the growth of mycoplasma. The colonies on the agar surface can also be used for fluorescent antibody staining and observation with an epifluorescence microscope. The advantage of this method is that it can use the colonies that originally grew on the agar surface without having to pass the mycoplasma.
Serological diagnostic test: enzyme-linked immunosorbent assay (ELISA) is highly sensitive: microimmunofluorescence (MIF) is fast.
3. Genetic diagnosis: The use of DNA probes to diagnose mycoplasma is slightly less sensitive, but has high specificity. Polymerase chain reaction (PCR) is used with high sensitivity