ND-2000 is the most widely used microscopic spectrophotometer in laboratories at home and abroad, and its superior performance and accurate results have won the praise of the majority of users.
This product can be used in the following aspects:
* Ultraviolet detection: detect the absorption value of the sample at conventional ultraviolet wavelength;
* nucleic acid detection: it can detect the concentration of different types of nucleic acids such as dsDNA, ssDNA and RNA and their absorption values at 260nm and 280nm;
* Probe detection: detect the light absorption value of fluorescent labeled probes, which can be used to remove samples that fail to label probes;
* Cell culture detection: detect the absorbance of cell culture at 600nm;
* Protein detection: detect the concentration of common purified proteins and the absorbance at 280nm;
* protein marker detection: protein samples calibrated by BCA, Bradford or Laurie can be detected, and the standard curve can be drawn automatically to calculate the protein concentration to be detected.
Second, product introduction
NanoDrop 2000 ultraspectrophotometer is the latest product of nanodrop. Applying the surface tension characteristics of liquid, the sample volume only needs 0.5~2ul. On the detection platform, the fixed optical path is pulled out by the contact of the upper and lower arms, which realizes the advantages of fast, trace, high concentration, quartz tube-free, capillary-free and other consumables to detect the absorption value. This product design is protected by patent and is very popular all over the world. Its accuracy and convenience enable scientists to conduct various kinds of research more effectively.
NanoDrop 2000 can provide full spectrum detection of 190~840nm by using pulsed xenon flash lamp, and it can be used after being turned on without preheating. With a highly sensitive CCD array detector, the detection absorption value can be as high as 300Abs(dsDNA concentration 2~ 15000ng/ul), and most purified nucleic acids can be detected almost without dilution.
The uniformity of the sample to be tested is the highest requirement of NanoDrop 2000. Generally, vortex mixer is the best way to shake nucleic acid and protein samples before testing. If there is no vortex mixer, use a pipette to mix evenly for many times. If you are worried that the genomic DNA may be broken due to the above actions, then change it to 55? C heating for about one minute, so that the sample presents a uniform state before detection, so as to ensure that 2ul is representative.
Choosing different detection modes in the detection process can get the fastest results:
● nucleic acid? C absorption spectrum, absorption values at 230 nm, 260 nm and 280 nm (converted into optical absorption value of 10mm), ratio of 260/280 of 260/230 and nucleic acid concentration.
● UV-Vis？ The absorption values and spectra of all wavelengths between C 190~840nm (expressed as the optical absorption value of 1mm).
● A280 protein quantitative method? C 280nm absorption value (converted into 10mm optical path absorption value), 260/280 ratio and protein concentration. It is only applicable to the purified protein, and can only be calculated when the mass extinction coefficient is known.
● BCA, Bradford and Laurie? C, automatically drawing a standard curve and calculating the r square according to the results of different wavelength absorption values provided by different color developers, and determining the protein concentration.
* protein detection mode currently provides three commonly used quantitative chromogenic reagents: BCA, Bradford and Lori. As the chromogenic reagent will gradually deepen with the passage of time, please read the reagent instructions carefully and prepare the standards with different concentrations before use, and complete the detection in the best time to get a good standard curve. Each standard curve can have up to seven standards, and each standard can be repeated up to five times.
* When measuring protein, the sample volume needs to be 2ul. After each sample is tested, it needs to be wiped 15~20 times with Kimwipes low-dust wiping paper (No.:34 155) to avoid the residue of developer and protein.
Concentration range:
Sample type
Minimum concentration
Maximum concentration
(Please dilute the sample when it exceeds)
Reproducibility (repeated more than five times)
nucleic acid
2 ng/microliter
15000 ng/l (dsDNA)
12000 ng/l (ribonucleic acid)
When the concentration range is 2- 100 ng/microliter, the standard deviation is 2 ng/microliter.
When the concentration range is greater than 100 ng/microliter, the coefficient of variation is 2%.
Pure bovine serum albumin
0. 10 mg/ml
100 mg/ml
When the concentration range is 0.05- 10 mg/ml, the standard deviation is 0. 10 mg/ml.
When the concentration is greater than 10 mg/ml, the coefficient of variation is 2%.
Protein, measure with BCA method.
0.2 mg/ml
8.0 mg/ml
CV: 2% (within the detectable concentration range)
Protein, quantified by improved Lowry method.
0.2 mg/ml
4.0 mg/ml
CV: 2% (within the detectable concentration range)
Protein, quantitative in Bradford way.
100 μ g/ml
8000 μ g/ml
When the concentration range is 100-500 ug/ml, SD is 25 ug/ml.
When the concentration is more than 500 μ g/ml, the coefficient of variation is 5%.
Protein, measure by mini Bradford.
15 μ g/ml
100 μ g/ml
When the concentration range is 15-50 ug/ml, SD is 4 ug/ml.
When the concentration is more than 50 μ g/ml, the coefficient of variation is 5%.
Third, the technical parameters:
1, wavelength range:190-840 nm;
2. Wavelength accuracy:1nm;
3. Resolution:
4. Others: 1mm optical path length (which can be automatically adjusted to 0.05 mm);
5. detection limit: 2 ng/μ l (dsdna);
6. Detection limit:15000 ng/μ l (dsdna);
7. absorbance accuracy: 0.002 absorbance (1 mm optical path);
8. Accuracy of absorbance: 2% (0.76 at 257 nm);
9. Absorbance range: 0.02-300 (equivalent to 10mm optical path);
10, nucleic acid detection cycle:
1 1, volume: 14cm×20cm.
Fourth, the use of examples:
DsDNA: Click on the nucleic acid on the main screen, and the computer and instrument will be automatically connected. Prepare the solution according to the solution for dissolving DNA (ensure that DNA is dissolved in secondary water, TE buffer or elution buffer of which kit), take out the 1.5 ul point on the detection platform, put down the upper arm and press blank. Select the sample type and DNA-50 in the upper right, enter the sample name in the sample ID position, mix the samples evenly, take out the 1.5 ul point on the detection platform, put down the upper arm, and press the measurement again.
Verb (abbreviation for verb) results sort:
The NanoDrop2000 software will ask you where to save the file at the beginning of the test. If not specified, the file will be stored in the file of the previous user.
Six, matters needing attention:
1. Wipe the countertop with a mirror wipe immediately after testing. First, take a piece to suck up the liquid on the upper and lower countertops, then fold the surface of the sample sucked out by wiping in the laboratory back inside for four times, and then wipe the countertops several times in one direction (DNA wiping 5 times, protein wiping 20 times).
2. The same drop of liquid can only be detected once. If you want to repeat the quantification of the same sample, please wipe off the previous drop and take out another drop for testing.
3. Nucleic acid samples can basically be determined by 1~2ul, and there will be different volume requirements according to the volume characteristics of each liquid, which should not exceed 2ul in principle. Please use 2ul pipette to avoid incomplete liquid column formation due to insufficient volume. Due to the characteristics of chromogenic agent and protein itself, only protein samples must be tested by 2ul.
4. When the software jumps out of the error message, please read it carefully and remove the obstacles according to the instructions. The most common situation is that the liquid column is not formed correctly in the detection process, and the following information will appear in the software. First, visually observe whether the liquid column is not completely connected to the upper and lower tables or whether there are bubbles in the sample. After pulling up the upper arm, wipe off the dripping sample and re-test. If necessary, increase the sample size to 2ul.
5. Do not use corrosive samples containing hydrofluoric acid (HF), and other non-corrosive liquids can be used.
Brand:
Nano-droplet 2000c
This instrument integrates all the functions of NanoDrop 2000 and the functions of the cuvette.
Advanced micro-pedestal technology
Dual Mode-Select a cuvette or substrate.
Wider concentration range, can measure very low or high concentration.
The function of the cuvette can be measured by kinetics (time or time/temperature study) and cell culture (OD 600).
Heating: 37 degrees Celsius, 0.5 degrees Celsius
Stirring: 150 to 850 rpm
Z height: 8.5mm.
Size of cuvette:12.5mm x12.5mm, with a maximum of 48mm.
Optical paths: 10, 5, 2 and1mm.
Type: shielded cuvette
Absorption range: 0.008 to 1.5.
Measurement time: < 3 seconds
Weight: 2. 1kg
UL/ csa and CE