Objective: Use Escherichia coli β-galactase gene LacZ as a reporter gene to establish transgenic mice containing loxp sites in Tyro3 and Axl receptor tyrosine kinase. Cross with Cre mice with different tissue specificities. , to obtain transgenic mice that express Tyro3 and Axl spatiotemporally, laying the foundation for studying their functions in various tissues. Methods The Tyro3 and Axl receptor tyrosine kinase plasmids containing loxp sites were constructed. After sequencing was correct, the plasmids were Injection method: Inject the transgenic vector of Geo-STOP-msAxl and Geo-STOP-msTyro3 genes downstream of the CAG promoter into fertilized eggs of BDF1 donor mice, transplant the fertilized eggs into ICR recipient mice, and carry out double-primer PCR after delivery. The genotypes of F0 and Fn generation mice were identified, RT-PCR, X-gal staining was used to observe the LacZ gene in mouse tissues, and WB (Western Blot) was used to detect the difference in the expression of the target protein in transgenic mice and wild-type mice. Results Transgenic positive mice containing the target gene were obtained; the presence of the LacZ gene was confirmed in the positive mice by RT-PCR; X-gal staining was performed on the mice with the LacZ gene, and the results showed that the brain, testis, and thymus of Geo-STOP-msTyro3 Blue precipitates were observed in the brain, heart, and kidneys of Geo-STOP-msAxl, indirectly indicating the tissues and organs in which the target gene can be expressed; for the double-positive Geo-STOP-msTyro3, ??Geo-STOP -msAxl transgenic mice and wild-type mice, WB test confirmed that there is no difference in the expression of Tyro3 and Axl genes, indicating that after adding the stop sequence, the Tyro3 gene does exist and the expression is inhibited. Conclusion Geo-STOP-msAxl and Geo -STOP-msTyro3 tyrosine receptor transgenic mice.