Solid-liquid two-phase medium Septi-Check AFB two-phase medium is an earlier medium used abroad [1], which is designed by closed solid-liquid two-phase integrated medium patented by BD. The liquid phase is a special enrichment medium for Mycobacterium zhongxi 7H9, which can rapidly propagate Mycobacterium. The solid phase consists of three solid medium planes: Middle brook 7H 1 1 and improved L-J medium, which are used to separate and purify mycobacteria in enrichment solution in time and obtain single colony. Chocolate agar is used to find contaminated bacteria at an early stage to avoid wasting time. Because the liquid phase is the foundation, Mycobacterium tuberculosis grows faster and is also a very effective culture medium. The Pleurotus ostreatus biphasic culture medium [2] prepared by China Pleurotus ostreatus is a kind of culture medium based on Pleurotus ostreatus leachate, which is prepared by adding calf serum, agar and other components, and is made into liquid and solid culture medium according to the amount of agar. It is rarely used in China, and its main characteristics are low cost and simple preparation, which is suitable for grass-roots use and has certain research value.