Since its birth, magnetic microspheres have attracted the attention of researchers and have been successfully applied in the field of biochemical analysis. In recent years, magnetic microspheres coated with specific antibodies, receptors and single-stranded DNA have been used to separate targets in complex samples and achieved great success. Compared with traditional separation methods, magnetic microspheres can be used to separate biochemical samples with complex components, which can realize separation and enrichment at the same time, greatly improving separation speed and enrichment efficiency, and also greatly improving the sensitivity of analysis and detection. At present, magnetic microspheres have been widely used in immunoassay, nucleic acid separation and extraction, cell sorting, enzyme immobilization and other fields. Recently, it has been reported in related articles that magnetic microspheres have been used to detect trace microorganisms or some active chemicals in environmental samples, and good results have been achieved.
Generally, magnetic microspheres used for biochemical analysis must meet the following conditions:
1) superparamagnetism means that it can quickly aggregate in the presence of a magnetic field, and can be uniformly dispersed in the absence of a magnetic field, and there is no aggregation phenomenon;
2) Appropriate particle size and narrow particle size distribution range make microspheres have strong magnetic response and will not settle due to excessive particle size;
3) The microspheres are rich in surface active groups, which can be coupled with biochemical substances and separated from the sample to be tested under the action of external magnetic field.
Superparamagnetic microspheres can be used in the following directions:
Cell classification
Protein purification
Nucleic acid extraction
Bacterial detection
Immunoprecipitation reaction
Immunochromatographic test
Biosensor
biochip
Shanghai Aorun Micro Nano New Materials Technology Co., Ltd. focuses on the research and development of magnetic nano-materials and their biomedical application technologies and products, and has a number of patented technologies independently developed, including serialized magnetic nano-spheres, magnetic nano-particles, biological application kits and supporting instruments and equipment, providing brand-new, efficient and fast tools and solutions for the separation and purification of nucleic acids, protein and cells, labeling of cells and biomolecules, gene transfection, rapid early diagnosis of diseases and magnetic resonance imaging diagnosis.
Oates? Brief introduction of superparamagnetic nanospheres
Oates? Superparamagnetic nanospheres are brand-new magnetic bead products produced by our company's unique patented technology. It is the only new magnetic microsphere with high magnetic content (above 70%), monodisperse controllable particle size, uniform core-shell structure and high specific surface area in the world, and its diameter ranges from nanometer to submicron. It not only avoids the shortcomings of low magnetic content and small specific surface area of traditional large-diameter microspheres, but also overcomes the shortcomings of slow magnetic response speed of small-diameter microspheres. Good particle size characteristics ensure the consistency of surface physical and chemical properties, and provide a guarantee for obtaining high-quality and reliable biological separation, purification and detection results.
At present, the company has formed Austrian magnetism? Superparamagnetic silica nanoparticles and Austrian magnetism? Two series products of superparamagnetic polymer nanospheres.
Superparamagnetic silica nanospheres are specially designed for nucleic acid purification. These nanospheres have a core-shell structure, that is, a high magnetic core and an inorganic silica shell, with a large number of silanol groups attached to the surface and suspended in water. In a specific solution environment, nanoparticles can quickly separate nucleic acids from the materials to be separated. It can be applied to the purification of various nucleic acids, such as extracting chromosomal DNA, bacterial chromosomal DNA, viral DNA/RNA, PCR fragments and plasmid DNA from blood. The purification of nucleic acid by magnetic beads is an important direction of the development of nucleic acid purification methods, because it does not need centrifugation and various reagents, and it is simple to operate and meets the requirements of automatic nucleic acid extraction.
superiority
The average particle size of microspheres is small and the specific surface area is large;
High magnetic content and fast magnetic response;
The binding amount of DNA/RNA per mg of magnetic microspheres is as high as 65438 0.2 μ g;
Excellent colloidal stability;
Good redispersibility.
Superparamagnetic polymer nanoparticles have the characteristics of monodispersity, uniform particle size distribution, high colloidal stability and superparamagnetic response. A series of different chemical functional groups on the surface of microspheres can combine with protein and ligands, which can be used in protein purification, affinity chromatography, cell classification and screening, immunoassay, clinical diagnosis and other biological fields. It is one of the indispensable separation and purification tools in medical and molecular biology research.
superiority
The microspheres have small particle size and large specific surface area;
High magnetic content and fast magnetic response;
High binding capacity;
Excellent colloidal stability;
Good redispersibility;
The surface of functionalized microspheres is suitable for coupling various biological probes;
The target product can be quickly separated from complex substances and keep its biological activity.