ApplicationNo. Application Date: August 25th, 2000.
Name: establishment technology of super male fish breeding system
Gong Kai (Announcement)No.: CN 1340298 Publication (Announcement) Date: March 20, 2002.
Main classification number: A01K6100 Original application number:
Classification number: A0 1K6 1/00.
Release Date: Priority:
Applicant: Wuhan Kangxiang Technology Development Co., Ltd.
Address: Wuhan Kangxiang Technology Development Co., Ltd., High-tech Entrepreneurship Center of Hongshan District Science and Technology Commission, Wuhan City, Hubei Province, 430070.
Inventor (designer): Chen Hongxi; Cui; International application of Guohai:
International publications: Entry date:
Patent Agency: Wuhan University of Water Resources and Electric Power Patent Office Agent: Ling Xue.
abstract
The invention discloses a technology for establishing a super male fish breeding system, which comprises the following steps: injecting a sealing agent with a certain concentration into a living body to generate non-meiotic homologous or heterologous diploid sperm; The nuclei of androgenetic blastocysts fertilized by homologous or heterologous diploid sperm and inactivated eggs are injected into mature unfertilized eggs to directly develop into homologous or heterologous super male fry, and some super male fry are transformed into physiological "females". Finally, the super male fish mated with the physiological "female fish" and hatched normal 100% super male fish offspring. The invention is of great significance to the artificial cultivation of new fish species, the production and cultivation of male fish in fishery scale and the protection of germplasm resources.
Claim 1. A technique for establishing a breeding system of super-male fish is characterized in that the selectively mature male fish is injected with oxytocin according to a conventional method, and a cell division blocker with a certain concentration is injected into the body to generate non-meiotic diploid sperm; At the same time, non-meiotic heterodiploid sperm were obtained by the same treatment; In addition, the mature unfertilized eggs are irradiated into ovum nucleus inactivation eggs, and the non-meiotic homologous or heterodiploid sperm are fertilized with the ovum nucleus inactivation eggs to obtain embryos in the blastocyst stage of androgenesis; Then, the nuclei of diploid androgenetic blastocysts are injected into mature unfertilized eggs by electrofusion or nuclear transfer technology, and normal super male fry are hatched, and some super male fry are transformed into physiological female fish by hormone treatment, and finally the super male fish is mated with the physiological female fish for production. Thus the breeding system of super male fish was established.