Generally speaking, the antibodies used in western recognize the amino acid sequence specificity, which is about 5-6 amino acids. After denaturation, the spatial structure of the protein changes, but its primary structure does not. Changed; the antibodies that can be used in ELISA depend on the type of antigen, some recognize amino acid sequence specificity, and some recognize conformation specificity. Therefore, it is best to determine based on the antibody instructions. In addition to the above mentioned, SDS in WB has another factor: low concentration of SDS has the effect of promoting protein solubility, stabilizing antibodies, and as a surfactant, it can fully infiltrate the NC membrane. The principle of this washing powder is similar. .