Immediate centrifugal saline test technology: can be used to detect saline-reactive antibodies that interfere with ABO stereotypes, but this method will detect antibodies that have no clinical significance and miss the detection of antibodies of IgG nature.
Low-ionic medium method: This method can be effectively used to detect irregular antibodies (IgG properties). The disadvantage of this method is that it cannot eliminate the interference of clinically significant antibodies.
Enzyme test (pineapple or papain): rarely used for antibody detection in pre-transfusion tests because enzymatic methods destroy some red blood cell antigens such as M, N, S, Fya and Fyb, but Enzyme technology can enhance the antibody detection of Rh and Kedd systems. Mainly used for antibody identification of MNS system.
Low-ionic polybrene technology: requires less test time (3 to 5 minutes), and the method is highly sensitive. However, since the reaction temperature is room temperature, the interference of clinically non-significant antibodies cannot be ruled out (especially at room temperature) saline reactive antibodies).
Polyethylene glycol-indirect anti-antibody method (PEG-IAT): PEG-IAT is a very sensitive and effective method for detecting antibodies, which can increase the intensity of the reaction. At the same time, this method can effectively avoid No clinically significant antibody interference.