The application number is 0 18 19083.
Date of application: 200 1-09- 19.
In the present invention, a polynucleotide with a structure capable of forming a loop is used as a template, and a plurality of primers capable of providing a complementary strand synthesis starting point for the loop are used in combination, so that isothermal and rapid polynucleotide synthesis becomes a reality. If the LAMP method is used, since the template polynucleotide itself can also be synthesized by isothermal reaction, all the reactions can be carried out quickly at the same temperature.
Legal status: rights
2. Tell me how to synthesize polynucleotides.
The application number is 0 18 19083.
Date of application: 200 1-09- 19.
In the present invention, a polynucleotide with a structure capable of forming a loop is used as a template, and a plurality of primers capable of providing a complementary strand synthesis starting point for the loop are used in combination, so that isothermal and rapid polynucleotide synthesis becomes a reality. If the LAMP method is used, since the template polynucleotide itself can also be synthesized by isothermal reaction, all the reactions can be carried out quickly at the same temperature.
Legal status: invalid