In order to meet the demand of rapid detection in the field, hand-held ATP fluorescence detector (also called hand-held ATP fluorescence detector) should be selected to realize real-time detection of flow state, because large desktop ATP fluorescence detector may be better than hand-held ATP detector in sensitivity, but it can only be used in laboratory, which is not conducive to on-site detection in mobile places such as catering service and medical control.
ATP detection technology abroad has been studied since 1990s, and has made great progress and development so far.
The experimental results of ATP detection technology are accurate, reliable and credible. Over 65,438+0,000 samples have been studied by many professional authorities in the United States. This rapid detection method can complete the detection in just over ten seconds, and the consistency between the experimental results and the results obtained by 24-48 hours standard bacterial culture method/plate method is over 90%. When detecting drinking water, the sensitivity is more than 100 times higher than that of the standard method, and the sample size is only1100 of that of the traditional method. Compared with the results obtained by traditional bacterial culture, the correlation of this product is as high as 92-98%. The Canadian Food Inspection Association found that the correlation between the determination results of this product and the standard culture method was 98%. FDA recommended ATP detection system and kit for rapid detection of bacteria in milk. The first Sino-US seminar on food safety and its whole process control and the training course on rapid automatic microbial detection technology also took ATP rapid detection technology as one of the training contents. At the 1 1 World Disinfection Conference and the 7th International Disinfection and Hospital Infection Control Forum held in Brazil, the participating experts made a research report on the application of ATP method in the evaluation of cleaning efficiency. The conclusion is that the effect of this method is basically the same as that of the standard culture method, so ATP method is included in the relevant standards of "medical cleanliness evaluation" in many countries as one of the evaluation methods. Through application and evaluation, the "Guidelines for Disinfection and Sterilization of Medical Institutions 2008" formulated by CDC in the United States also uses ATP method as a new method to evaluate the effect of endoscopic cleaning or endoscopic treatment.
In recent years, due to the domestic application demand, leading units have developed hand-held ATP fluorescence detectors and ATP reagents with independent intellectual property rights, which have been successfully applied in many fields.
China CDC has confirmed that ATP test results are linearly related to different concentrations of Escherichia coli, Staphylococcus aureus and blood. Through the application and technical evaluation of ATP method for bacteria detection and surface cleanliness detection, ATP fluorescence detector has become a special detection equipment related to health supervision and food safety designated by China's health supervision and China's food and drug supervision official department, and has been designated by the state for rapid detection of surface cleanliness of objects and operators in food processing, storage and transportation, trade, catering service and medical system, and it is an important measure for building the capacity of health supervision and rapid detection in the field of food safety to purchase ATP fluorescence detector and supporting reagents.
In China, the development of hand-held ATP fluorescence detector began around 2005. On 20 10, the first hand-held ATP fluorescence detector and an integrated swab for on-site express detection of ATP reagents were developed, and the corresponding invention patent for the first hand-held ATP fluorescence detection system in China was obtained. With the expansion of demand and the gradual maturity of products, the strong sales growth of domestic instruments has gradually replaced the share of imported instruments.
◆ Since 2005, the research and development of hand-held ATP fluorescence detector and its supporting reagents have been started.
In 2006, he undertook the Xi science and technology plan project "Rapid detection system and instrument for harmful pathogens based on ATP fluorescence and pyrophosphate sequencing".
◆ In 2007, the handheld ATP fluorescence detection system applied for a patent, and in 2009, it obtained the national invention patent authorization certificate.
◆ In 2007, the hand-held ATP fluorescence detector was supported by the National Technology Innovation Fund for Small and Medium-sized Science and Technology Enterprises, and passed the acceptance on 20 10.
◆ In 2007, the inspection report of the quality inspection agency was obtained, and the performance reached and exceeded the indicators specified by relevant departments.
In 2008, it won the national key new product certificate.
◆ In 2009, exports began to earn foreign exchange.
◆2065 438+00 won the second prize of Shaanxi Patent and the second prize of xi Science and Technology.
◆20 10 won the silver award in the National Development Exhibition.
◆2065 438+02 On-site rapid integrated detection of ATP reagent obtained the national patent authorization.
◆ By 20 10, BioLum portable ATP fluorescence detector and on-site rapid ATP detection integrated swab will be put into mass production, and the performance will meet and exceed the requirements of national standards, meeting the requirements of food and drug supervision departments, health supervision departments, food processing industries, catering services, aviation catering, large enterprise canteens, school canteens, large-scale activity support departments and other food safety-related fields, such as surface detection of bacteria and environmental objects, hand hygiene detection, and so on. Bacterial detection and surface cleanliness detection in pollution supervision fields such as cosmetics manufacturing pollution control, hospital infection control, air bacterial pollution detection, and environmental supervision departments.
◆20 1 1 year and 20 12 years to meet the needs of the above-mentioned national health supervision and food and drug supervision departments and replace imported products.
◆20 12 with the support of the national 863 science and technology plan and Shaanxi science and technology innovation project plan, we have further studied ATP fluorescence detectors with higher performance, such as medical water quality detection and effect evaluation of medical devices after cleaning.