(1) Azotobacter is sensitive to soil pH, and its optimum pH is 7.0 ~ 7.5. When applying nitrogen-fixing bacterial fertilizer on acid soil, lime should be used in combination to improve nitrogen fixation efficiency. Overacid, overbased fertilizers or fungicidal pesticides should not be mixed with nitrogen-fixing bacteria fertilizers to avoid strong inhibition.
(2) Nitrogen-fixing bacteria have high requirements for soil moisture. When the soil moisture content is 25% ~ 40% of the maximum field capacity, they begin to grow, and when the soil moisture content is 60% ~ 70%, they grow best. Therefore, attention should be paid to soil moisture conditions when applying nitrogen-fixing bacterial fertilizer.
(3) Nitrogen-fixing bacteria are mesophilic bacteria, and the optimum growth temperature is 25 ~ 30℃. When it is lower than 10℃ or higher than 40℃, its growth will be inhibited. Therefore, nitrogen-fixing bacteria fertilizer should be stored in a cool place and keep a certain humidity to prevent exposure to the sun.
(4) Nitrogen-fixing bacteria can only play a full role in nitrogen fixation in an environment rich in carbohydrates and lacking in bound nitrogen. When the ratio of soil carbon to nitrogen is lower than 40 ~ 70 ∶ 1, the nitrogen fixation stops rapidly. The suitable ratio of carbon to nitrogen in soil is the most important condition for nitrogen-fixing bacteria to develop into dominant bacteria to fix nitrogen. Therefore, it is best to apply nitrogen-fixing bacteria or organic fertilizer on soil rich in organic matter.
(5) After applying a large amount of nitrogen fertilizer to the soil, apply nitrogen-fixing bacteria fertilizer every 10 day, otherwise the nitrogen-fixing ability of nitrogen-fixing bacteria will be reduced. The combined application of nitrogen-fixing bacteria with phosphorus, potassium and trace element fertilizers can promote the activity of nitrogen-fixing bacteria, especially in poor soil.
(6) Nitrogen-fixing bacterial fertilizer is suitable for all kinds of crops, especially Gramineae crops and leafy vegetables. Nitrogen-fixing bacteria fertilizer is generally used for seed dressing, mixed sowing, and then covered with soil to avoid direct sunlight. It can also be dipped in roots or used as a base fertilizer for vegetable seedbeds, or mixed with cotton seed fertilizer. It can also be applied to crop roots or combined with irrigation.