Thereby inducing malignant tumors. Therefore, the detection of clenbuterol in meat must be controlled from the source. At present, the detection methods are as follows:
Gas chromatography-mass spectrometry
The advantage of GC-MS method is that it organically combines the high-efficiency and rapid separation effect of chromatography with the high-sensitivity qualitative analysis of mass spectrometry, and can be used for qualitative and quantitative analysis of a specific residue in the presence of multiple residues, with a high detection limit. Fanta company. A et al. used GC-MS to detect the residue of CLB in cow hair, and the minimum detection limit was 5 ng/g; The content of CLB in cattle, sheep and pigs was detected by gas chromatography-tandem mass spectrometry (GC-MS-MS), and the minimum detection limit was 2 ng/g; Liu Qi et al. used GC-MS (EI ion source) to detect CLB in pig urine, and the detection limit was 0.5ng/mL. VanRhijin et al. used trimethylsilyl or 2- dimethylsilyl morpholine derivatives to detect CLB in urine extract, and the derivatives were scanned by electric pulse or chemical ionization, which would produce high sensitivity. In addition, compared with HPLC, GC-MS has higher detection sensitivity and lower false positive rate. Therefore, GC-MS method is considered as the confirmation method for detecting CLB in China (NY/T468~200 1).
High performance liquid chromatography (HPLC)
HPLC is suitable for the determination of thermally unstable and strongly polar β -agonists and their metabolites. Moreover, HPLC can be combined with pre-column extraction, purification, post-column fluorescence derivatization and mass spectrometry, which is easy to realize the automation of analysis process. Huang Shixin et al. (1995) used an ultraviolet detector to detect CLB residues in pig liver and pork. The detection condition was λ=243nm, the chromatographic column was Shimpakklc-ODS150× 6.0 Mn, the flow rate was 1mL/min, and the column temperature was room temperature: 30. Some people abroad use HPLC (diode array detector) to determine CLB residues in animal food. The minimum detection limit is 1.26ng/g, and the recovery rate is 98.9%. At present, high performance liquid chromatography (HPLC) has been used as a semi-confirmatory method to detect CLB residues in China. The minimum detection limit is 1 ~ 15 ng/g, which has the advantages of good specificity, strong selectivity, high detection accuracy and low false positive rate. The disadvantage is that the sample processing time is long, the detection process is cumbersome and difficult to operate, and expensive instruments are needed, which is limited in practical application.
Enzyme-linked immunosorbent assay (ELISA)
Using the specific binding of immune antigen and antibody and the efficient catalysis of enzyme, plant horseradish peroxidase (HRP) was chemically combined with clenbuterol (CL) to form enzyme-coupled clenbuterol. Loading solid phase