What is virus preservation solution?

Virus preservation solution is suitable for the collection, preservation and transportation of common virus samples such as viruses, influenza viruses, hand, foot and mouth viruses. It is a liquid that protects the tested substances of viruses by soaking the swab virus samples in the sampling tube. Throat swabs, nasal swabs or tissue samples from specific parts can be collected, and the stored samples can be used for subsequent clinical experiments such as nucleic acid extraction or purification. Usually divided into two types, one is the non-inactivated type, which can protect the protein and nucleic acid of the virus, and the other is the inactivated type, which usually contains lysis salts that inactivate the virus, cleavage the protein and protect the nucleic acid. 1. Inactivated type: The virus preservation solution with added lysis salt is the inactivated virus preservation solution. The main purpose of containing Tris, lysis salt, EDTA, etc. is to cleave nucleic acid and release the nucleic acid, so that through subsequent real-time fluorescence RT -PCR performs nucleic acid testing to determine whether the sample contains viral signature nucleic acid, that is, whether it is infected with the virus. Nucleic acid is a biological macromolecular compound composed of many nucleotides, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). It is one of the most basic substances of life. The virus has a single structure and contains only one kind of nucleic acid and protein. Therefore, the virus is detected when the characteristic nucleic acid is detected. Viruses are parasitic organisms and cannot survive outside the body after sampling. If they cannot be detected in time, they need to be placed in a virus preservation solution. In order to protect the safety of the virus detection environment, lysis salt needs to be added to inactivate the virus and release nucleic acids that can be detected. 2. Non-inactivated virus preservation solution: In addition to the inactivated virus preservation solution, there is also a non-inactivated virus preservation solution, which does not contain lysis salt, but the integrity of the preserved virus is better and the detection rate is higher. In addition to nucleic acid detection It can also be used for other research. Inactivated virus preservation solutions are safer to use, and the operating environment requirements are not so strict. Each has its own advantages. The components of the non-inactivated virus preservation solutions currently studied are mainly based on Hank's solution. Based on Hank's solution, Various components such as gentamicin, fungal antibiotics, BSA (the fifth component of bovine serum albumin), biological buffer HEPES, amino acids, cryoprotectants, glycerin, etc. are also added