To evaluate the quality of a technology, we mainly look at its rapidity, specificity, and sensitivity.
Compared with conventional PCR, there is no need for processes such as thermal denaturation of the template, temperature cycling, electrophoresis, and UV observation. Loop-mediated isothermal amplification is a new nucleic acid amplification method that is simple, fast, and highly specific.
This technology is comparable to or even better than PCR technology in terms of sensitivity, specificity and detection range. It does not rely on any specialized equipment to achieve on-site high-throughput rapid detection, and the detection cost is much lower than fluorescence quantification. PCR.
Extended information:
Amplification principle:
The specific process of the LAMP technology amplification reaction is relatively complicated, as shown below in conjunction with Figure 2, according to the specific The reaction process is explained in detail on the LAMP principle.
In the startup phase, the double-stranded DNA template anneals under isothermal (60-65°C) conditions, and the F2 sequence in FIP binds to the Flc site on the template strand (2).
Nucleic acid synthesis is initiated under the action of DNA polymerase with strand displacement activity (2), and the external primer m also slowly anneals and hybridizes with the target sequence (3), initiating the strand displacement reaction to generate a double stranded DNA (4), and displaces to release a single-stranded DNA (5); one end of the single-stranded DNA automatically forms a loop structure.
Baidu Encyclopedia-Loop-Mediated Isothermal Amplification Technology