Chromatographic columns can be divided into analytical and preparative types according to their uses
①Conventional analytical columns (constant columns), inner diameter 2~5mm, column length 10~30cm;
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②Narrowbore column, inner diameter 1~2mm, column length 10~20cm;
③Capillary column (also called microcolumn), inner diameter 0.2~0.5mm;
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④ Semi-preparative column, inner diameter >5mm;
⑤ Laboratory preparation column, inner diameter 20~40mm, column length 10~30cm;
⑥Inner diameter of production preparatory column Up to tens of centimeters.
Extended information
The performance of the chromatographic column is not only related to the performance of the stationary phase, but also related to the packing technology. Under normal conditions, when the packing particle size is >20um, dry packing is more suitable to prepare the column; when the particle size is >20um, wet packing is more ideal. There are generally four filling methods:
① High-pressure homogenization method, which is mostly used for filling analytical columns and small-scale preparation columns;
② Radial pressure method, patented by Waters;
③Axis pressure method, mainly used for filling large-diameter columns;
④Dry method, column filling is highly technical, and most laboratories use packed commercial columns .
It must be pointed out that filling technology is an important part of obtaining high-performance liquid chromatography columns, but the fundamental problem lies in the performance of the filler itself and whether the structure of the supporting chromatograph system is reasonable.
Baidu Encyclopedia-Liquid Chromatography Column