Common expression vectors for eukaryotic cells
1. pCMVp-NEO-BAN vector
Features: The molecular weight of this eukaryotic cell expression vector is 6600 base pairs. It is mainly composed of CMVp promoter, rabbit β-globin gene intron, polyadenine, ampicillin resistance gene and anti-neo gene, and pBR322 skeleton. It can stably express exogenous genes at high levels in most eukaryotic cells. Target gene.
2.pEGFP, enhanced fluorescent protein expression vector (Enhanced Fluorecent Protein Vector)
Features: pEGFP expression vector contains green fluorescent protein, in the PCMV promoter Driven by high-level expression in eukaryotic cells. The SV40 origin in the vector backbone allows the vector to replicate in any eukaryotic cells expressing SV40 T antigen.
3. pEGFT-Actin, enhanced green Fluorescent protein/human actin expression vector
Features: The pEGFP-Actin expression vector contains green fluorescent protein and human cytoplasmic β-actin genes, driven by the PCMV promoter, in eukaryotic cells Medium- and high-level expression. The SV40 origin in the vector backbone allows the vector to replicate in any eukaryotic cell expressing the SV40 T antigen.