Using the floral organs of four varieties, Dibo, Siberia, Tiepao and Golden Lily, as explants, the tissue culture difficulty test of flower organs of different varieties and different parts of flower organs of the same variety was conducted. The results show that there are differences in induction rates among varieties. The order from easy to difficult is Dibo, Siberia, Tiepao, and Golden Lily. There are also differences in the induction rate of different parts of the same variety. The order from easy to difficult is filament, receptacle, Ovary, corolla, style, anthers. In the primary induction medium, MS+6-BA 0.5m g/L+NAA 0.5m g/L and MS+6-BA 1.0m g/L+NAA 0.5m g/L are better; in the secondary proliferation medium Among them, the optimal culture medium for both Dibor and Siberia was M S+6-BA 1.0m g/L+NAA 0.2m g/L, while the optimal culture medium for Tiegun Lily was M S+6-BA 1.0m g/L+NAA 0.5m g/ L. ..