When Qian Yongjian was a graduate student at Cambridge University in England, he invented a better dye to track the intracellular calcium level. Calcium plays a key role in many physiological reactions, including nerve impulse regulation, muscle contraction and fertilization. However, at that time, the method of measuring intracellular calcium level was quite primitive, and calcium-binding protein needed to be injected through the cell wall, which usually destroyed the research cells.
Later, Qian Yongjian invented dye molecules to detect the concentration of calcium ions. 198 1 year, he improved the method of introducing dyes into cells, and later invented more and better dyes, which were widely used. There are three methods to detect calcium: selective electrode method, jellyfish method and calcium dye method.
Before the appearance of calcium dyes in Qian Yongjian, only jellyfish had the ability of space detection, but at that time jellyfish needed to be injected into cells, which was inconvenient to use, while dyes in Qian Yongjian could penetrate into cells. Jellyfish and calcium dyes have their own advantages and disadvantages, and many people are using dyes at present.
Qian Yongjian later began to study GFP, improving its luminous intensity and color (inventing variants and many different colors), inventing more application methods and clarifying its luminous principle. Most FP used in the world are variations of his invention. His patent was used by many people and sold by the company.
1962, Shimomura and Johnson wrote a footnote in the article "Purification of Jellyfish", saying that another kind of protein was discovered, which was green in sunlight, yellow in tungsten filament irradiation and strong green in ultraviolet radiation. Later, they carefully studied its luminous characteristics.
Therefore, Qian Yongjian's invention has made great contributions to the field of chemical research, and I hope that more people will devote themselves to scientific research in the future to make our motherland stronger.