The following is its instruction manual
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Chapter 1 Overview 1.1 Principle The principle of spectrophotometric analysis is to use substances Qualitative and quantitative analysis of substances is carried out through the phenomenon of selective absorption of light of different wavelengths. Through the analysis of absorption spectra, the structure and chemical composition of substances can be judged. This instrument works based on the principle of relative measurement, that is, a certain solvent (distilled water, air or sample) is selected as the reference solution, and its transmittance (ie, transmittance T) is set to 100, while the test sample The transmittance of is obtained relative to this reference solution. The change in transmittance (transmittance T) has a certain functional relationship with the concentration of the measured substance. Within a certain range, it conforms to Lambert-Beer's law. T=I/I. A=KCL=-㏒ I/Io where T is transmittance (transmittance) A absorbance C solution concentration K absorbance coefficient of solution L length of liquid layer in the optical path I light passes through the test sample and then irradiates onto the photoelectric converter The intensity Io is the intensity of light that passes through the reference test sample and irradiates onto the photoelectric converter. UNICO UV-2600 series UV-visible spectrophotometer is developed based on this principle, combined with modern precision optics and the latest microelectronics and other high technologies. A new generation of advanced spectrophotometer with international advanced level. 1.2 Usage It can be used for scientific research in physics, chemistry, medicine, biology, pharmacology, geology and other disciplines. It is widely used in chemical industry, pharmaceuticals, biochemistry, metallurgy, light industry, materials, environmental protection, medical testing and other industries and analysis industries. One of the most important quality control instruments in the laboratory and an essential instrument for routine laboratories. 1.3 Features UV-2600 series UV-visible spectrophotometer has the following features: Using a low stray light, high-resolution single-beam optical path structure monochromator, the instrument has good stability, reproducibility and accurate measurement readings. There are fixed slits of 4nm or 1.8nm and variable slits of 0.5nm, 1nm, 2nm, 4nm, etc. for you to choose from to meet the monochromatic bandwidth requirements of different analysis and test projects. Using the latest microprocessor technology, the instrument not only has control functions such as automatic setting of 0T and 100T, as well as concentration calculation and data processing functions for various methods, but also has special functions to prevent users from operating errors, so there is no need to worry when using it. Scientific design and the application of new technologies organically combine optical, mechanical, electrical and microcomputer technologies, making the instrument's stability index close to or reaching the level of advanced UV-visible spectrophotometers. The large-screen graphic LCD can not only display data, but also can display maps. The rich in-machine software can complete quantitative analysis, qualitative analysis, kinetics, multi-wavelength, DNA/Protein and other tests, coupled with powerful storage and printing function, all testing, analysis and data output can be completed without connecting to a computer. The instrument can also optionally be equipped with UNICO user application software that can run on the Win9x operating platform, giving the instrument greater functionality.
1 Chapter 2 Main technical indicators 2.1 Technical indicators Main technical indicators of UV-2600 series UV-visible spectrophotometer Model UV-2600 UV-2600A Optical system Single beam, 1200 lines/mm Diffraction grating Spectral bandwidth Wavelength range Wavelength accuracy Wavelength repeatability Wavelength Resolution Data Display Stray Light Photometric Range Photometric Accuracy Stability Baseline Linearity Data Output 4nm 190nm-1100 nm ±0.8nm 0.5nm 0.1 nm 320×240 Graphic LCD ≤0.05T 0-200T at 220nm, 340 nm, -0.3 -3.0A, 0-9999C (0-9999F) ±0.3T ±0.002A/hour @500nm (after preheating for 1 hour) ±0.002A (after upgrading to scanning type) USB port (used to connect to PC, cannot be connected Printer) Centronics parallel port can be used with various HP, Epson and Lenovo laser and inkjet printers (see Appendix A). It can also have a built-in micro printer (optional). 2nm Dimensions (mm) Power consumption weight 530×390×280 80W (220V 3.15A insurance Φ5×20) 14.5kg 2.2 After unpacking the random accessories, please carefully check whether the following items on the packing list are complete: Packing list item name Spectrophotometer............. ................................................................. Power cord........................................ ..................Cuvette ............................. .................................. Quantity 1 1 4 Glass ................................................................. ............. 2 Quartz 2 1 Dust cover ............................. ..................................User Manual............. ................................................................. .. 2.3 The appearance of the instrument is shown in Figure 1, Figure 2 Built-in printer cover Sample chamber cover LCD display 1 1 Keyboard Colorimetric slot lever Figure 1 Light source observation hole Fan power socket Power switch Fuse Figure 2 3 Printing port USB port LCD contrast adjustment 2.4 Instrument Installation 1. After unpacking, carefully check whether the items in the box are complete and intact according to the packing list; 2. Place the instrument on a horizontal platform. The instrument should avoid direct sunlight, away from electromagnetic transmitting devices and high-power electrical devices, and use the environment There should be no dust, corrosive gas and vibration; 3. There should not be any obstacles around the instrument that affect the flow of air around the instrument; 4. Use the power cord provided by UNICO and confirm that the power socket has a good ground wire; 5. Connect the instrument It must be warmed up for at least 20 minutes before testing.
Chapter 3 Basic Operation of the Instrument 3.1 Display and Keys The following figure is a schematic diagram of the display and keys 3: UV-2600 Display and Keys Diagram 3 4 Key Description LOAD SAVE SETλ λ 0Abs/100T PRINT START ESC/STOP ENTER F1-F4 0-9 /-/. CLEAR ＜, ＞ ＜ ＞ ∧, ∨ ∧ ∨ CELL Data recall key; Data storage key; Set wavelength key; Adjust 100T/0Abs, and establish user baseline key; Printout key; Test or test Start key; Return to the front screen display or cancel the current operation; Input confirmation key; Function keys correspond to the display on the screen; Number and letter keys; Positive and negative signs and decimal points clear the screen, clear the current input data, and delete files; Modify X coordinates, observe the data point by point; modify the Y coordinate, observe the peak points point by point, enter uppercase and lowercase letters to change; set the position of the sample slot (when an 8-channel rack is installed). 3.2 Power on the instrument Power on the instrument (each time After powering off, do not power on immediately, wait at least 10 seconds), and let the instrument warm up for at least 15 minutes before testing. Note: 1. After powering on, the instrument will automatically self-check and initialize. First check the memory (Figure 4). Press any key to skip this step. After the initialization is completed, the instrument will warm up for 15 minutes (Figure 5). When 15 minutes is up or press ESC/STOP to skip to Figure 6, the bottom line of the screen will display: Recalibrate the system? No (Figure 6), select "Yes" To do system calibration (recommended to select Yes in Figure 7), select " "No" to skip., three beeps, and enter the main display interface (Figure 8); 2. If the data in the memory is lost, the instrument will calibrate the system directly. If the data in the memory is lost, the instrument will calibrate the system directly. 3. If the instrument does not have an automatic sample rack installed, "Sample Rack #1" in Figure 8 will not be displayed. will not be displayed. Wavelength: 656.1nm Starting EastRTOS, please wait for memory detection... Detection memory: 16kb D2 W Unocal (Shanghai) Instrument Co., Ltd. Spectro-Quest Figure 4 Note: If 15 minutes cannot be automatically skipped, it is in the machine Caused by the clock stopping, please press ESC/STOP to manually skip and set the clock in "System Settings", see 10.1.4 5 Figure 5 Figure 6 Figure 7 Figure 8 6 3.3 Basic operation of the instrument 3.3.1 Adjust the blank so that the cuvette containing the reference solution enters the light path; press the 0Abs/100T key to adjust the blank. Note: 1. If the reference solution is too thick, "Insufficient energy..." will be displayed on the upper right corner of the screen (Figure 9). If "If the reference solution is too thick, the amount is too low..." will be displayed on the upper right corner of the screen corner, the test will be aborted, and the warning symbol "Warning..." will be displayed in the center of the screen (Figure 10). 2. If the automatic sample rack is not installed, in Figure 10, "Sample Rack #1" and "Max E" will not will appear. Figure 9 Figure 10 3.3.2 Set the wavelength. The steps to set the wavelength in the "photometer mode" are as follows: Press the SETλ key (Figure 11). λ 7 Figure 11 A dialog bar will appear in the lower part of the screen (Figure 12). Use the numeric keys to enter the desired value. Wavelength 450nm. Figure 12 Press ENTER key to confirm.
The wavelength goes from 656.1nm to 450.0nm, and then automatically adjusts to blank once. The final screen display is as shown in Figure 13. Figure 13 3.3.3 Retrieve, store, and print experimental results a. For example, the steps to retrieve the curve in "Spectrum Scan" are as follows : Press the LOAD key. The bottom line of the screen will display the first file ABC.wav in the memory (Figure 14). At this time: 1. Press the ∧ key or ∨ key to view the files in the memory ∧ ∨ 2. Press the ENTER key to load the current file into the screen (Figure 15). Just note that the extension of the selected file must be wav. Otherwise, it will display: "File type error...". Store under various tests The extensions corresponding to the files are shown in Table 1. 3． Press the CLEAR key, the bottom line of the screen will display "Are you sure? No". Press the ∧ ∧ key or ∨ key, the bottom line of the screen will show "Are you sure? Yes". At this time, if you ∨ 8 press ENTER to confirm, it will be cleared. Remove the currently selected file. Figure 14 Table 1 Experimental quantitative measurement Standard curve Quantitative measurement Experimental results Spectral scan kinetic measurement DNA/protein measurement Multi-wavelength measurement Storage file extension ***.fit ***.qua ***.wav ***.kin * **.dna ***.mul The steps to save the curve in "Spectrum Scan" are as follows: Press the SAVE key. The lowest line of the screen displays "Please enter a file name?" Use the numeric keys to enter letters or numbers such as: XYZ (Figure 15 ), press ENTER to confirm the save. Note that the file name can be up to three characters long. Note: 1. Press the numeric keys continuously (the interval between two key presses is less than 0.5 seconds) to enter letters or characters. ∧ Press the ∧ key or ∨ key to change the case of letters. The characters corresponding to the numeric keys are shown in Table 2. ∨ Table 2 Characters that can be represented by numeric keys Characters that can be represented by numeric keys Characters that can be represented by numeric keys 0 0, ,-,*,/1 1,#,?,:,I 2 2,A,B,C,= 3 3, D, E, F, 4 4, G, H, I, { 5 5, J, K, L, } 6 6, M, N, O, ~ 7 7, P, Q, R, S, 8 8, T, U, V, " 9 9, W, X, Y, Z /-/. -,., 2. If the entered file name has the same name as a stored file, the lowest line of the screen displays The file has the same name, are you sure? No." Press the ∧ or ∨ key, the bottom line of the screen will display "The file ∧ ∨ has the same name, are you sure? Yes." If you press ENTER to confirm, the previous file with the same name will be replaced. Cover. Note: 1. The capacity and reliability of the memory used to store experimental results are not as good as those of a PC. Therefore, it is strongly recommended to print or upload important experimental data to a PC in time to avoid losing your It may cause unexpected trouble; 2. The memory in the instrument is the same as the hard disk memory of the PC. Repeated access will cause the efficiency to decrease and even become inaccessible. It is strongly recommended to clear it regularly, that is, format it. See 10.4. 9 Figure 15 c. Printing the experiment report Press the PRINT key in the "Photometer Mode" Figure 16 to print out the test results as shown in Figure 17 Figure 16 Press the PRINT key to print the report (Figure 17). Figure 17 3.4 Preparation before the test: Clean the test cuvette or test tube with distilled water or other special cleaning agents, and use a soft cotton cloth or paper towel to wipe away any fingerprints or drops on the surface; Place the cuvette into the slot closest to you in the 4-link manual sample rack, then push the push rod forward to make the cuvette face the light path, and close the sample chamber cover; Chapter 4 Photometer Mode UV-2600 Series Spectrophotometer The meter provides users with a variety of different analysis methods. The photometer mode is the most basic test mode.
4.1 Description of test method. Push the reference solution into the optical path. Press the 1 button in the main menu in Figure 7 to enter the "photometer mode test interface. Enter the photometer mode". After entering the photometer mode, the instrument will automatically blank once, and then the screen will display as shown in Figure 18. If the instrument is equipped with an automatic sample rack 10, the screen will be displayed as shown in Figure 19. Further operations can be performed. If you press ESC/STOP, you will return to the main menu. Note: If the instrument is not equipped with an automatic sample rack, the "sample rack #1" and " Max E" will not be displayed. Figure 18 Figure 19 By pressing F2, there are three test modes to choose from, namely: absorbance, transmittance, and content. Figure 20 4.1.1 Absorbance mode reference liquid enters the optical path. After pressing F2, press ∧ or ∨Select absorbance mode, press ENTER ∧ ∨ to confirm, press 0Abs/100T to calibrate the blank, and finally pull the test sample into the light path and read the test results (Figure 20). 4.1.2 Transmittance mode reference liquid enters the optical path. Press F2 and then press ∧ or ∨ to select the transmittance mode, press ENTER ∧ ∨ to confirm, press 0Abs/100T to calibrate the blank, and finally pull the test sample into the optical path and read Get the test results. . 4.1.3 Content (concentration) mode Press F1 and then ∧ ∨ to select the concentration unit, ENTER ∧ or ∨ to confirm (Figure 21). 11 If there is no concentration unit you need, you can select "Custom" and press After ENTER confirmation, customize the concentration unit by entering numbers or letters, and then press ENTER to confirm, Figure 22. Figure 21 Figure 22 Enter the reference liquid into the light path and press 0Abs/100T to adjust the blank. Next, there are two methods to measure the concentration: a. Press F3 to directly enter the value of the known concentration factor F, and press ENTER to confirm, Figure 23. Then Pull the solution to be tested into the optical path and read the concentration value; b. Pull the standard solution with a known concentration value into the optical path, press F4 to enter the concentration value of the standard solution and press ENTER to confirm, Figure 24. Then pull the solution to be tested into the optical path Read the concentration value; Note: 1. To select a wavelength, you can press SETλ at any time and enter the wavelength value and then press λ ENTER to confirm. After the wavelength is selected, the instrument will always automatically adjust to blank once; 2. If the concentration factor is If the value F is greater than 9999, a "data out of limit" message will be displayed. Figure 23 12 Figure 24 4.2 Print the experiment report. Press PRINT to print the experimental results as shown in Figure 25. Figure 25 Press 2 in the main interface of quantitative measurement in Chapter 5 to directly enter the "Quantitative Measurement interface" as shown in Figure 26. Press ESC/STOP to return to the main interface. "Quantitative Measurement" Quantitative Measurement interface. Note: If the instrument is not equipped with an automatic sample rack, "Sample Rack #1" and "Max E" will not be displayed. Figure 26 5.1 Description of measurement method 5.1.1 Select concentration unit 13 Press F1 to select concentration unit, Figure 27, the method is as described in 4.1.3.
Figure 27 5.1.2 Select the calibration method Press SETλ to select the calibration method. The UV-2600 series spectrophotometer provides three calibration lambda methods to choose from, namely: single wavelength method, equal absorption point double wavelength method and three wavelength method. Figure 28. Note: For the introduction of the three methods, please refer to Appendix C. Figure 28 5.1.3 Select the curve fitting method. Press F2 in Figure 26 to enter the display interface of Figure 29. Figure 29 Press 1 to select the fitting method. There are four types. Methods for you to choose: first-order linear fitting, first-order linear fitting through 14 zero, second-order fitting and third-order fitting. 5.1.4 Directly input the standard curve in Figure 29, press F2 to directly input a standard Curve, shown in Figure 29A. Figure 29A Note: The number of factors entered is related to the selected curve fitting method. The following table is the corresponding relationship: Curve fitting method The first order of factors required for the curve equation expression Linear zero-crossing fitting C=K1×A K1, r* First-order linear fitting C=K0 K1×A K0, K1, r* 2 Second-order fitting C=K0 K1×A K2×A K0, K1, K2 2 3 Third-order fitting C=K0 K1×A K2×A K3×A K0, K1, K2, K3 * r is the linear regression correlation coefficient 5.1.5. Establish a standard curve. In Figure 29, press the F3 key to pass the test. A set of standard samples is used to establish a standard curve. See Figure 30. a. Use the numeric keys to directly enter the concentration value of the standard solution. Press the ∧ or ∨ key to choose to modify the ∧ ∨ input concentration value of the standard solution, see Figure 31. Press ESC/STOP to end this modification and exit. Figure 30 15 Figure 31 b. After the reference solution is pulled into the optical path, press 0Abs/ 100T, the instrument will go to the selected wavelength (it may be single wavelength, dual wavelength or triple wavelength depending on the selected calibration method) and adjust the blank. Figure 32 shows. Figure 32 Pull each standard sample into the light path one by one and press the START key to measure the A value of the standard sample step by step, as shown in Figure 33. Figure 33 c. Press the F4 key to draw a curve. At this time, you can press the F1 key to select different fitting methods to obtain different fitting curves, as shown in Figure 34, Figure 35, Figure 36, and Figure 37. Note: If the number of samples is small, select the second order, especially the third order. Order curve fitting will give invalid results. 16 Figure 34 First-order linear zero-crossing fitting figure 35 First-order linear fitting figure 36 Second-order fitting figure 37 Third-order fitting 17 At this time, press the PRINT key to print out the curve, and press the ESC/STOP key to return to the previous level. interface. Then, press the SAVE key and name the curve to save it. 5.1.6 Quantitative measurement The first step is to obtain the standard curve. There are three methods to obtain the standard curve for quantitative measurement, which are described below: Note: Quantitative measurement should be performed under the display interface in Figure 26. a. Load the standard curve stored in the machine and perform testing. Press the LOAD key in the display interface shown in Figure 33. Press the ∧ or ∨ key to select the file with the suffix ∧ ∨ extension ***.fit and press ENTER to confirm the import. . Then, press the ESC/STOP key to return to the front-end interface (Figure 26) for testing. b. Use the known standard curve to conduct the test. Press the F2 key in the display interface shown in Figure 33, and then directly enter the coefficients of the standard curve. Then, press the ESC/STOP key to return to the front-end interface (Figure 26) for testing. Note: Before inputting the standard curve, you must select the fitting method by pressing F1 based on the known standard curve. For example, if the known standard curve is a second-order curve, you must select second-order fitting. c. Use the newly established standard curve to conduct experiments. As mentioned in 5.1.5 above, a standard curve has been established. Press the ESC/STOP key to return to the previous interface (Figure 26) to conduct the experiment.
The second step, after pulling the reference solution into the light path, press the 0Abs/100T key to adjust the blank. The third step, after pulling the sample to be tested into the light path, press the START key, and the test results will be displayed on the screen. Figure 38 shows. Figure 38 The fourth step is to print, store and call up the test results. Press the PRINT key to print out the test report. Figure 39 shows. 18 Figure 39. Press the SAVE key in Figure 38, enter the file name and press ENTER to confirm. Complete storage. In Figure 38, press LOAD, then press the ∧ or ∨ key to select the file with the suffix extension ***.qua ∧ ∨, and press ENTER to confirm loading the saved file. Chapter 6 Spectrum Scanning Note: UV-2600 Basic Type None If you need this function, please contact our sales department. (Telephone 021-64955137) Press 3 in the main interface to directly enter the "spectrum scan interface as shown in Figure 41. Press ESC/STOP Spectrum Scan" Spectrum Scan returns to the main interface. Figure 41 6.1 Parameter Settings Press F1 to set the scan parameters. Including the scan start wavelength, end wavelength, scan interval and scan speed. Press the ∧ or ∨ key to select the Y-axis scale, as shown in Figure 42. ∧ ∨ 19 Figure 42 Note: (1) Since the instrument always sweeps from high wavelength to low wavelength, set the starting wave of the scan. Since the instrument always sweeps from high wavelength to low wavelength, set the length to be greater than the end wavelength; (2 ) The scan interval can only be 0.1nm, 0.2nm, 0.5nm, 1nm, 2nm and 5nm. Each scan can process up to 3000 data points, so when the scan range is set large, the scan interval cannot be set too small. ; (3) The scanning speed is "high speed", "medium speed" and "low speed". 6.2 Scan mode selection. Press F2 in Figure 41 to select the measurement mode, "Abs", "T" and "E" "Three modes are available, as shown in Figure 43. Figure 43 6.3 Establishing the baseline After pulling the reference liquid into the optical path, press the 0Abs/100T key to adjust the blank to establish the baseline (Figure 44). The ESC/STOP key can stop scanning ; Press 20 Figure 44 6.4 Scan the sample After pulling the sample to be analyzed into the light path, press the START key to scan the sample. During the scanning process, press the ESC/STOP key to stop scanning (Figure 45). The buzzer will sound three times when the scanning is completed. Figure 46 shows. Figure 45 Figure 46 6.5 Spectrum processing 6.5.1 Change scale After scanning, press the ＜ or ＞ key to change the X-axis scale, and press the ∧ or ∨ ＜ ＞ ∧ ∨ key to change the Y-axis scale, as shown in Figure 47, Figure 48 shown. Figure 48 is only a part of Figure 47. 21 Figure 47 Figure 48 6.5.2 Peak and valley query Press the F3 key to enter the interface shown in Figure 49 and perform peak and valley search. The instrument design has two types of searches for you to choose from: Figure 49 a. Search point by point: Press the > key to search point by point from left to right, and press the < key to search point by point from right to left> <. The search step size is consistent with the scan interval. The retrieved data is shown on the first line of the display. . b. Point-by-point peak and valley search: Press the ∧ key to perform a peak and valley search point by point from left to right, and press the ∨ key ∧ ∨ to perform a peak and valley search point by point from right to left. The retrieved data is also displayed on the first line of the display. . Figure 51 shows. 22 Figure 50 Figure 51 Note: In Figure 49, press F1 F1 key to set the search height of point-by-point peak and valley search. The smaller the value, the more peak and valley points are retrieved, and vice versa.
6.5.3 Store, load and print the scanning curve a. As shown in Figure 46, the scanning spectrum of a certain sample has been completed. Press the SAVE key, enter the file name and press ENTER to confirm. b. In Figure 41, Press the LOAD key, then press the ∧ or ∨ key to select the file with the suffix extension ∧ ∨ named ***.wav, and press ENTER to confirm loading the saved scan curve. c. Press the PRINT key in Figure 46 to print out the scan curve. As shown in Figure 52. 23 Figure 52 24 Chapter 7 Dynamic Measurement Note: The UV-2600 basic model does not have this function. If you need this function, please contact our company's sales department. (Telephone 021-64955137) Press 4 in the main interface to directly enter the "Kinetics Measurement Interface as shown in Figure 53. Press Kinetic Measurement" ESC/STOP to return to the main interface for kinetic measurement. Figure 53 7.1 Parameter settings are shown in Figure 53 Press F1 in the display interface to set the test parameters, including total running time, delay time and time interval. Press the ∧ or ∨ key to select the Y-axis scale, as shown in Figure 54. ∧ ∨ Figure 54 7.2 Press F2 to select the measurement mode Select the test mode, "Absorbance" mode or "Transmittance" mode, as shown in Figure 55. 25 Figure 55 7.3 Measurement steps a. Press SETλ to select the test wavelength. After pulling the reference liquid into the light path, press 0Abs/100T λ Key blank. b. Pull the sample to be tested into the light path and press the START button to start time scanning of the sample. During the scan, press the ESC/STOP button to abort the scan. When the scan is completed, there will be three buzzer sounds, as shown in Figure 56. Figure 56 7.4 Reaction rate calculation after the experiment. You can press the F3 key to calculate the kinetic reaction rate, enter the time values ??for the calculation starting point and end point, and the value of the calculation factor F and press the ENTER key to confirm. The reaction rate can be calculated, as shown in Figure 57 and Figure 58. Note: I.U. = F 6.5.2 7.6 Store, load, and print experimental results a. As shown in Figure 58, the kinetic curve of a certain sample has been completed. Press the SAVE key, enter the file name and press ENTER to confirm, and the storage of the curve is completed. b. In Figure 53, press the LOAD key, then press the ∧ or ∨ key to select the file with the suffix extension ∧ ∨ ***.kin, and press ENTER to confirm loading the saved kinetic experimental curve. c. Print kinetics Press the PRINT key in Curve 58 to print out the kinetic experimental curve, as shown in Figure 59. 27 Figure 59 Chapter 8 DNA/Protein Measurement Protein Measurement Note: UV-2600 basic model does not have this function. If you need this function, please Contact our sales department. (Telephone 021-64955137) Press 5 in the main interface to directly enter the "DNA/protein measurement interface as shown in Figure 60. Press Protein Measurement" ESC/STOP Protein measurement returns to the main interface. Note: Regarding the specific details of DNA/protein measurement and protein measurement For the algorithm, please refer to Appendix A. Protein Measurement 28 Figure 60 8.1 Parameter setting Press F1 to select calculation factors f1-f4, as shown in Figure 61. The default values ??of calculation factors have been installed in the machine, but the user is allowed to input different calculations. factor. Figure 61 8.2 Select the measurement mode Press the F2 key to select the measurement mode. After the "Absorbance Difference 1" mode or "Absorbance Difference 2" mode is selected, select whether to "Measure Background".
The measurement wavelengths of "Absorbance Difference 1" mode are 260nm and 280nm, and the background wavelength is 320nm (optional). The measurement wavelengths of "Absorbance Difference 2" mode are 260nm and 230nm, and the background wavelength is still 320nm (optional). Figure 62, Figure 63. Figure 62 29 Figure 63 8.3 Select the concentration unit and press the F3 key to select the concentration (content) unit (Figure 64). Figure 64 8.4 Measurement steps a. Pull the reference liquid into the light path and press the 0Abs/100T key to adjust blank. b. Pull the sample to be measured into the light path and press the START key to start measurement. The final measurement results are shown in Figure 65. Figure 65 c. If there are multiple samples to be tested under the above settings, just press the START key again. 30 d. Press the ＜ or ＞ key to view the test results of multiple samples, and directly enter the sample ＜ ＞ number that is Yes, for example 3, as shown in Figure 66, you can also press the ∧ and ∨ keys to view the test results one by one ∧ ∨. Figure 66 8.5 Restore parameter default values ??If the test parameters have been modified, including modifications to the calculation factors f1-f4 or modifications to the test wavelength and background wavelength, press the F4 key to restore them. 8.6 Store, call out and print test results a. In Figure 65, press the SAVE key, enter the file name and press ENTER to confirm to complete the storage of the test results. b. In Figure 60, press the LOAD key, and then press ∧ or Press the ∨ key to select the file with the suffix extension ∧ ∨ for ***.dna. Press ENTER to confirm and recall the saved test results. c. In Figure 65, press the PRINT key to print out the test report, Figure 67. Figure 67 31 Chapter 9 Chapter 9 Multi-wavelength measurement In the main interface, press 6 to directly enter the "multi-wavelength measurement interface as shown in Figure 68. Press ESC/STOP Multi-wavelength measurement" Multi-wavelength measurement returns to the main interface. Figure 68 9.1 For parameter setting, press the F1 key to enter the wavelength input editing interface. After inputting the wavelength, press ENTER to confirm (picture key to enter the wavelength input editing interface, 69). Press the ∧ or ∨ key to enter more wavelengths. Press the CLEAR key to clear the entered wavelength. Press the ESC/STOP key to exit this interface. key to exit this interface. Note: It is recommended that the maximum wavelength be entered first. Figure 69 9.2 Select the measurement mode. Press the F2 key to select the measurement mode, as shown in Figure 70. 32 Figure 70 9.3 Measurement steps a. Pull in the reference solution In the light path, press the 0Abs/100T key to adjust the blank. b. Pull the sample to be measured into the light path, and press the START key to start measurement. After measuring a set of wavelengths, it always returns to the first wavelength. The final measurement results are displayed as shown in Figure 71. Figure 71 c. If there are multiple samples to be tested under the above settings, just press the START key again. d. Press the ＜ or ＞ key to view the test results of multiple samples, directly Just enter the sample ＜ ＞ product number, or press ∧ and