1. Routine maintenance:
1. The appearance of the sterilizer and the sterilization chamber must be kept clean and dry.
2. The probe and water level gauge should be cleaned regularly.
3. The door frame and rubber ring are not damaged, and the steam inlet must not be blocked. It is best to apply talcum powder to the strip after daily use. To extend the life of the strip.
4. The door interlocking device must be flexible, reliable, and open freely.
5. Clean the drain valve once a month to facilitate the discharge of cold air and maintain temperature.
2. Maintenance cycle:
After one year of use, a qualified testing department must be asked to conduct a comprehensive and systematic inspection every year. Including: cylinder, door, piping system, electrical system, etc. Safety valves, temperature gauges, and pressure gauges must be calibrated regularly to ensure the safety and normal use of the equipment.
3. Operation steps:
1. First, take out the inner sterilization bucket, and then add an appropriate amount of water to the outer pot so that the water surface is level with the triangular shelf. should.
2. Return the sterilization bucket and load the items to be sterilized. Be careful not to pack it too tightly, so as not to hinder the flow of steam and affect the sterilization effect. The mouth ends of the Erlenmeyer flask and the test tube should not be in contact with the wall of the barrel to prevent condensation water from soaking the paper wrapping the mouth and penetrating into the cotton plug.
3. Add a cover and insert the exhaust hose on the cover into the exhaust slot of the inner sterilization barrel. Then tighten the two opposite bolts at the same time in a symmetrical manner to make the bolts tight and tight to prevent air leakage.
4. Use an electric stove or gas to heat, and at the same time open the exhaust valve to boil the water to remove the cold air in the pot. After the cold air is completely exhausted, close the exhaust valve and let the temperature in the pot gradually rise as the steam pressure increases. When the pressure in the pot rises to the required pressure, control the heat source and maintain the pressure for the required time. This experiment used 1.05kg/cm2, 121.3℃, 20 minutes sterilization.
5. After the sterilization time is up, cut off the power or turn off the gas and let the temperature in the sterilization pot drop naturally. When the pressure on the pressure gauge drops to 0, open the exhaust valve and loosen the bolts. , open the lid and take out the sterilized items. If the pressure does not drop to 0 and the exhaust valve is opened, the pressure in the pot will suddenly drop, causing the culture medium in the container to rush out of the flask or test tube mouth due to the imbalance of internal and external pressure, causing the cotton plug to be contaminated with the culture medium. pollute.
6. Place the removed sterilized culture medium into a 37°C incubator and incubate it for 24 hours. After inspection, if there is no growth of foreign bacteria, it is ready for use.