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The application number is 201310201411.2.
Date of application: 20 13-05-27
Abstract: The invention belongs to the technical field of preparation methods of polypeptide drugs, and particularly relates to a preparation method of liraglutide. The technical problems to be solved by the invention are that the existing preparation method is difficult to separate and purify, and the total yield and purity of the product are low. The technical scheme for solving the technical problem is to provide a preparation method of liraglutide. The method comprises the following steps: preparing liraglutide resin through solid-phase polypeptide, performing acid hydrolysis to obtain crude liraglutide, and finally purifying to obtain pure liraglutide. The solid-phase polypeptide method includes the following steps: amino resin is connected with corresponding protective amino acids or protective amino acid fragments in the following sequence in turn through solid-phase coupling, and liraglutide resin is prepared: BOC-W (TRT)-X (OTBU)-THR (TBU)-PHE-THR (TBU)-SER (TBU)-ASP (OTBU)-VAL. -Glu (otbu)-phe-ile-ala-Trp (BOC)-leu-val-z (pbf)-arg (pbf)-Gly-resin; Where w is His-Ala, x is Glu-Gly, y is Nα-PAL-Glu, and z is Arg-Gly. The invention provides a new method for shortening the production cycle and improving the product purity and yield.
Applicant: Chengdu Shengnuo Biopharmaceutical Co., Ltd.
Address: 6 1 1330, No.258, Section 1, Yegong Avenue, Dayi County, Chengdu, Sichuan Province
Inventor (Designer): Guo Dewen Zeng Changliang Wen Yongjun.
Main classification number: C07K14/605 (2006438+0) i.
Classification number: c07k14/605 (2006.01) ic07k116 (2006.01) ic07k106 (.