Can a lawyer represent a colleague's spouse in a criminal case?

Yes, as long as the parties have no interest. According to the law, the following persons may not be entrusted as defenders:

(a) was sentenced to probation, the sentence has not been executed;

(2) A person who is deprived or restricted of personal freedom according to law;

(3) Persons without capacity or persons with limited capacity;

(4) Current employees of people's courts, people's procuratorates, public security organs, state security organs and prisons;

(5) People's jurors in our hospital;

(6) People who have an interest in the trial result of this case;

(7) Foreigners or stateless persons.