1. The sales volume of the double pest detection system of UniBest Company in the United States is higher than that of other manufacturers in the global market.
2. The double-worm elutriation system of UniBest Company in the United States adopts the elutriation mode of new technology, and thoroughly eluviates the collected filter bags through the natural elutriation mode, which not only eluviates the double-worm 100%, but also ensures the integrity of the double-worm.
3. The novel and unique filter bag structure can ensure that the two insects are completely intercepted and easily eluted, which is more suitable for treating high turbidity water samples and conforms to China's national conditions.
4. The disposable filter bag is designed to avoid cross-contamination and ensure the authenticity and effectiveness of the two-insect experiment.
5. The shelf life of the reagent is as long as 24 months, which can not only meet the testing needs of customers in the current year, but also ensure that the reagent is still effective in the next year's testing, thus making greater use of the reagent, avoiding the economic losses caused by the short shelf life of the reagent and saving costs for units and enterprises.
Second, the detection steps
GB 5750. 12- 2006 Giardia and Cryptosporidium in Standard Test Methods for Drinking Water
The detection steps are as follows: sampling and filtering → elutriation and concentration → immunomagnetic separation → fluorescence staining → microscope counting.
Step 1: Sampling and filtering?
Characteristics of filter capsules:
1, specially designed for eluting two kinds of insects, in line with Chinese national standard GB.
5749-2006, US Environmental Protection Agency, UK DWI and Australia TGA, etc.
2. The pore size is 0.45μm, and 100% intercepts two kinds of insects.
3, disposable, to avoid cross-contamination.
4, high pressure resistance
Step 2: elutriation and concentration
Equipment characteristics:
1, one-click automatic and rapid repeated elutriation of two insect samples collected.
2. The elutriation time only takes 2 minutes, and the whole experiment only takes 5 hours. There is no need for post-maintenance and maintenance. The world's light and fast elutriation equipment is suitable for emergency and laboratory operation.
3. The recovery rate of this equipment is as high as 90%, which is the two kinds of pest detection equipment with the highest recovery rate in the world at present.
Step 3: immune magnetic separation
In the process of immune magnetic separation, two kinds of special immune magnetic separation kits for worms, ClearScan series from UniBest Youbaida Company of the United States, were used to combine magnetic beads with specific antibodies with specific antigens of Giardia and Cryptosporidium in the sample, and the high-efficiency selective separation was carried out in the magnetic field, which can ensure the specificity of detection.
Step 4: Fluorescent staining/microscopic examination
The stained samples were detected by two kinds of special fluorescence microscopes for insects. The observed objects that met the size, shape and fluorescence characteristics of cysts and oocysts were qualitatively and quantitatively analyzed according to the characteristics of Giardia cysts and Cryptosporidium cysts in GB/T 5750. 12-2006.
Third, the technical parameters
1, and the recovery rate of filtration step ≥ 100%.
2. The recovery rate of filtration/elutriation step is ≥ 80%
3. The recovery rate of 3.IMS immunomagnetic separation step is ≥90%.
4. The total recovery rate (filtration, elutriation, magnetic bead separation and dyeing) is more than 35%.
5. The relative standard deviation of the test results is ≤ 10%.
6. The elutriation pressure is 0.5bar, and the elutriation step can be completed quickly without compressed air. This advanced elutriation method can not only ensure the complete elution of the two worms, but also ensure the complete morphology of the two worms.
7. detection of treatable high turbidity water samples: > 100 NTU
8. The kit is equipped with prefabricated positive control slides to ensure the effective activity of the kit; Completely avoid false detection and ensure the accuracy of detection.
9. Shelf life: The shelf life is as long as 24 months, and it is an IFA reagent with longer shelf life and more stable performance in the global industry.
10, the filter bag can be recycled and can withstand high pressure.
1 1. Water sample treatment capacity: water treatment capacity ≥ 1000L tap water each time; ≥ 100L source water
12. Upper limit of sampling flow rate: 4L/min.
13. Total time of elutriation step: 2 minutes.
14, filter module structure: including ≥ 100 porous reticulated foam layer with two different specifications: ≥60 large foam layer with an outer diameter of 42mm and an inner diameter of 34mm；; ≥40 small foam layers, with an outer diameter of 30mm, an inner diameter of 25mm and a thickness of 10mm, stacked alternately.
15, working voltage: 220V 10%(AC%), 50HZ.
16, working environment temperature and humidity: 5-40℃, 5%-95%.
Fourth, the system overview
The main performance and technical indicators of the system equipment, the use and characteristics of the equipment.
Elution
system
Automatic rapid elutriation system 1, one-button automatic rapid elutriation.
2. The elutriation time only takes 2 minutes, and the whole experiment only takes 5 hours, and there is no need for regular maintenance, which is suitable for emergency and laboratory operation.
The recovery rate of this equipment is 90%, which is the highest in the world at present.
Immune magnetic separation system UniBest company
Immunomagnetic separation kit for "two kinds of insects"
1, the program is simple, which greatly saves manual operation time.
2. Even if the content of two insects in each concentrated water sample is low, the recovery rate of magnetic separation can be kept above 90%.
3. It can be well matched with a new generation of automatic elutriation equipment, greatly improving elutriation efficiency and further improving the recovery rate of magnetic separation step.
1, passed the national standard GB5749-2006 and American EPA- 1623.
2. It can obviously improve the recovery rate of cryptosporidium and Giardia.
3. The background of residual debris can be reduced during microscopic examination.
4. Cysts/oocysts as low as 1 can also be detected.
UniBest company
Sample mixer
1. The mixing wheel can hold 12 test tubes at the same time.
2, the speed of 2-40 rpm is adjustable, with digital display.
Before being used in the step of immunomagnetic separation, the magnetic beads and oocysts are mixed evenly to make them fully combined, thus improving the recovery rate of the two worms.
Magnetic separator (miniature and large capacity) 1, and the working volume of large capacity magnetic separator: 5- 15ml, which is specially used for matching side flat wall test tubes.
2. Working volume of micro magnetic separator:1.5ml; ; Six standard 1.5ml microcentrifuge tubes can be placed simultaneously.
Magnetic separator is a special tool for immune magnetic separation of two kinds of insects. When the test tube is inserted into a magnetic separator to concentrate magnetic particles, the magnetic beads will be attracted to the test tube wall by the magnetic field. Therefore, supernatant and impurities can be removed smoothly and separated from magnetic beads and worms.
Side flat wall test tube The special side flat wall test tube for two kinds of insects is equipped with a large capacity magnetic separator, which is specially used for the immune magnetic separation experiment of two kinds of insects.
IFA staining method
system
IFA uniqlo
Indirect immunofluorescence? Dyeing kit
1, rapid and accurate detection.
2. The reagent is cost-effective, and the special fixing liquid is used, so the sample does not fade, and the fluorescence effect can be maintained for several days for re-inspection.
3. The shelf life of reagents is usually more than 24 months.
UniBest two-worm fluorescent reagent is a specific immunoglobulin M pentamer, which simultaneously labels an antibody through five FITC binding sites. It has remarkable bright fluorescence performance and keeps the fluorescence effect for a long time in the industry.
The standard dosage of quality control standard sample is 5 times that of positive control experiment, which makes the experimental results more accurate and reliable.
Microscopic observation
system
Fluorescence microscope 1, special fluorescence microscope for sampling two kinds of insects.
2, equipped with high-end imaging equipment, so that the imaging effect is obvious.
This microscopic detection system can well complete the bright field and dark field microscopic observation, and has good reliability and stability.