A, aseptic operation link
1, the inoculation room should be kept clean, the countertops and walls should be scrubbed with powdered phenol soap, and fumigated with lactic acid or formaldehyde regularly. Disinfect with ultraviolet lamp before each use; In the process of use, "Nicole" dynamic sterilizer is adopted. This machine is turned on when workers go to work, and it is not allowed to turn off when continuous sterilization is carried out. Workers turn off their power synchronously after work, which is harmless to human body, and can kill fungi, Bacillus subtilis, Salmonella and other miscellaneous bacteria in the air online from time to time.
2, before entering the inoculation room, should first do a good job of personal hygiene, in the buffer room to replace work shoes and hats, overalls, wearing masks. Work clothes, work shoes and masks are only allowed to be used in the inoculation room. Don't wear it elsewhere, change it regularly and disinfect it.
3. Inoculated test tubes, triangular bottles, etc. Should be marked, indicating the name and date of the culture medium and strain. All items moved into the inoculation room must be wiped clean with 70% alcohol in the buffer room.
4. Before inoculation, hands should be disinfected with automatic induction hand sterilizer. It is recommended to use 75% medical alcohol as disinfection medium. The process is as follows: "Wash your hands with an induction soap dispenser-rinse the faucet-induction drying-induction hand disinfection"; Based on the time after the first disinfection, the hand should be disinfected every 60-90 minutes to prevent the growth and reproduction of hand bacteria.
5. The incubator and inoculation tools should be cleaned and disinfected frequently; No alcohol lamp flame is left in the operation process; The tampon is not misplaced; Inoculation tools need flame disinfection before and after use.
Second, the air disinfection method of inoculation room
1, ultraviolet disinfection, its sterilization mechanism is that when it acts on organisms, it can lead to photochemical reaction between nucleic acids and enzymes in cells, leading to cell death. In addition, ultraviolet rays can also make oxygen in the air produce ozone, which has a bactericidal effect.
However, the effect on fungi is poor, and it only plays an auxiliary disinfection role, which needs to be used in conjunction with drugs. Because ultraviolet light is harmful to human body, don't work with ultraviolet light on.
2. The ozone generator can kill all kinds of microorganisms in the air and on the surface of objects efficiently and quickly, and the success rate of inoculation can reach over 97%; Meanwhile, it has the characteristics of stable performance, simple operation and low power consumption. Harmful to human body. Don't work in front of people
3. "Nicole Dynamic air disinfection machine" disinfects the air in the workshop to ensure food safety. When the workshop is disinfected by disinfection technology, people can work in the workshop at the same time without any harm to human body, and at the same time, the environment is controlled to maintain the standard of "sterility and dust-free".
4. Formaldehyde and potassium permanganate fumigation. The use method is to use 8 ~ 10 ml of 40% formaldehyde and 5 ~ 7 grams of potassium permanganate per cubic meter of space. Firstly, potassium permanganate is poured into a ceramic or glass container, and then formaldehyde is added; Leave immediately after adding formaldehyde, and seal the room for disinfection for 20 ~ 30 minutes.
Three, inoculation room sterility test method
Take sterile nutrient agar plates, one in the upper layer and one in the lower layer of the inoculation room, open the dish cover 15min, then cover it, and invert it at 37℃ for 24-28h. If the number of colonies in each Petri dish does not exceed 4, it can be considered that the sterility is good. If there are many colonies, the inoculation room should be further disinfected.
For the above information and "How to do a good job of sterilization before inoculation in aseptic inoculation room", if you have any questions, please consult "Hunan Innocent Purification Engineering Company".