When the peak shape is symmetrical, the chromatographic peak is regarded as an isosceles triangle. The area calculated by this method is 0.94 times of the actual peak area.
A = 1.064h×W0.5
Features: simple and fast.
Second, the peak height multiplied by the average peak width method:
When the peak shape is asymmetric, the peak width and peak area are measured at the peak height of 0. 15 and 0.85 respectively. The calculation formula is as follows:
A = h×(W0. 15 + W0.85)/ 2
Features: the result is correct and the measurement is troublesome.
Three, the peak height multiplied by the retention time method:
This method can be used to determine the peak area for narrow peaks and overlapping peaks (incomplete overlapping) whose half-peak width is difficult to measure. Calculation formula of peak area:
A = h×b×tR
Characteristics: The result is correct, which is suitable for the analysis of homologues (under certain operating conditions, the half-peak width of homologues is proportional to the retention time).
Four, the peak height multiplied by the peak width method:
When the peak shape is short and wide, the formula for calculating the peak area is:
A = 1.0204h×Wb
Five, automatic integrator method and chromatographic workstation method:
Measuring peak area is faster and more accurate than other methods, which can improve the degree of analysis automation.
Features: simple, fast and accurate.
Measurement of overlapping peak of intransitive verbs;
1, peak-cutting splitting method.
2. Vertical peak splitting method.
3. Connection peak method.
4. Computer fitting and peak splitting.