2. Monitor follicular development and determine the time for taking eggs. (with ultrasonic instrument)
3. Take eggs, transfer them into culture medium and fertilize them. (With the help of B-ultrasound)
4. Sperm collection: Sperm collection is carried out at the same time on the day of ovulation to optimize and screen excellent sperm.
5. In vitro fertilization and early embryo culture: the treated eggs and sperm are cultured in a culture medium to fertilize them in vitro. If the fertilized egg is formed and develops normally, it is transferred to a Petri dish containing growth liquid for further culture, and then blastocyst transfer is carried out.
6. Gene screening and blastocyst transplantation: On the fifth day, we can know the sex of the fetus, and on the sixth day, we will do PGS/PGD gene test, and put the well-developed blastocyst into the uterine cavity through the transplantation catheter, so that the blastocyst can successfully implant and continue to grow and develop in the uterine cavity.
7. Management after transplantation: Progesterone or HCG is given after transplantation to promote the proliferation of luteal cells, promote the synthesis and secretion of progesterone, and support luteal function.
8, confirm pregnancy test: after implantation 12 days, blood can be drawn to check whether it is pregnant; 2 days later, draw blood to check whether it is stable; Observe for 7 days after stabilization.