Mitochondria are organelles that exist in most cells and are covered by two membranes. It is the structure that produces energy in cells and the main place for cells to carry out aerobic respiration. It is called "Power House". Its diameter is about 0.5 to 65438 0.0 micron.
Except Entamoeba histolytica, Giardia lamblia and several microsporidia, most eukaryotic cells have mitochondria to some extent, but their own mitochondria are different in size, number and appearance.
Extended data
Points for attention in observation experiment:
1. In order to ensure the integrity of mitochondria during the whole operation, the operating environment such as temperature (0-4℃) and pH value (about 7.0) should be kept as constant as possible and the operating time should be as short as possible.
2. Tissue cultured cells should be carefully digested to prevent loss or duplication.
3. When homogenizing, the medium used must be isotonic buffer, and 0.25 mol/L sucrose solution or physiological saline is commonly used instead of Hank's solution.
4. The amount of homogenate depends on the tightness of homogenizer. Too few times and incomplete cell damage will affect mitochondrial production.
5. Therefore, 2/3 of the supernatant was used to prepare mitochondria to prevent excessive cell debris from affecting observation.
6. Generally, the whole separation process should be completed within 30-60 minutes, not too long.
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