1-2 guaiacol is produced by sheshan chemical plant with a chemical purity of ≥99%. The identification of true and false bamboo oil has always been an urgent problem to be solved when buying bamboo oil, and it has not been reported so far. Guaiacol is a representative effective component in bamboo oil, and its TLC is very clear. Under the experimental conditions, the Rf of guaiacol is 0.5s, and the iodine vapor star in bamboo oil. The stain of guaiacol is the only distinguishing feature of bamboo oil. For bamboo oil mixed with a large number of tea leaves or dilute brown sugar water, the actual content of bamboo oil is reduced so much that the guaiacol concentration of the same sampling amount is lower than the minimum detection limit. Therefore, when the tea leaves are mixed in the same position, there are almost no spots in bamboo oil and sugar water. As for tea, sugar water and bamboo ash leaching solution, there is no guaiacol at all. So there will be no chromatographic spots of guaiacol. Under 365nm ultraviolet light, tea leaves have strong blue fluorescent spots at Rf0.24, which are not found in bamboo oil, so it can be identified whether bamboo oil is mixed with tea leaves. For bamboo oil, there are blue fluorescent spots at RF 0.098, RFO.57 and Rio.90, which is also a distinguishing feature of bamboo oil.
Foam reaction is a unique physical property of bamboo oil, which may be related to the suspended state and certain consistency of bamboo oil, but the counterfeit and shoddy products of bamboo oil do not have this property. Tannin inversion is unique to tea and tea mixed with bamboo oil. Even tea contains a lot of tannin. When FeCI test solution is encountered, black precipitation of iron tannin is produced immediately. Fresh bamboo oil basically contains no tannin, but it is easy to occur with FeCl in bamboo oil. Straightening is very effective Through comprehensive identification, we can correctly distinguish the authenticity of bamboo oil and ensure the quality of fresh bamboo oil.