Hello, is it convenient to consult now?

1: Select the gene you want to see. If you don't specifically detect a gene, but want to observe a cell state or a cell, then find a gene that is specifically expressed in that state as the target gene. When you say white blood cells in rats, what white blood cells do you want to observe?

2. The introduction is generally to search for what has been reported in other people's articles first. Such a sequence is more reliable. As long as it is not very unpopular genes, most of them can be found. Of course, they can be found in English literature. If not, check the mRNA sequence of this gene, and then design a primer with an amplification length of about 200. Take the primer design software to choose one with a high score, and then take it to blast to see how specific it is, that is, whether this pair of primers can only amplify this sequence and not others.

3. Conventional fluorescence quantitative PCR does not need too much optimization, just use primers directly, and the so-called kit is just mixed use. If the result is not good, you need to adjust the conditions.