1.CHO-K 1: It is an unmodified wild-type CHO cell. Initially, the subclone of the original CHO cell line was preserved in ATCC by Puck and Kao's laboratory, about 1970, and named as. Subsequently, the subclone from ATCC CHO-K 1 was isolated in 1985 and stored in ECACC, which was used by pharmaceutical companies and CMO companies to express recombinant proteins.
2.CHO is an improved CHO cell, such as CHO-S, which is developed on the basis of the original CHO cell line. CHO cell which can be used for suspension culture was isolated by Thompson laboratory in 1973 and named CHO-S. This cell line was provided to Gibco Company in the late 1980s, and the company domesticated the cell into CD CHO medium, established a library and promoted it under the name of CHO-S.
When comparing the differences between the two cells, we should pay attention to their sources, characteristics, application fields and other related information besides whether they are transformed or not.