Content determination of Shijiazhuang Dongfang Pharmaceutical Co., Ltd..

According to high performance liquid chromatography (China Pharmacopoeia, 2000 edition, appendix ⅵ d). Chromatographic conditions and system applicability test with octadecylsilane bonded silica gel as filler; Acetonitrile-water (13:87) as the mobile phase; The detection wavelength is 230 nm. The theoretical plate number should be not less than 4000 calculated by paeoniflorin peak. Preparation of Reference Solution Precisely weigh the appropriate amount of paeoniflorin reference substance, add methanol to make a solution containing 0.02mg per 65438±0ml, and shake well to get the final product. Preparation of test solution Take the contents of different volumes, grind them evenly, accurately weigh 0.5g, put them in a triangular bottle with a stopper, accurately add 25ml of methanol, weigh them, perform ultrasonic treatment for 30min, cool them, make up for the weightlessness with methanol, filter them, accurately measure the continuous filtrate 10ml, put them in a water bath for drying, dissolve the residue with 15ml of water, and move to the. Adjust the pH value to 9 ~ 10 with ammonia test solution, extract with mixed solution of n-butanol and chloroform (1: 1) for 5 times, 30ml each time, combine the filtrates, dry in water bath, add methanol to the residue, quantitatively transfer to 10ml volumetric flask, add methanol to the scale, shake well, and use. The determination method accurately absorbs 65438 00μ l control solution and test solution respectively, and injects them into the liquid chromatograph for determination. The content of paeoniflorin (C23H28O 1 1) in each capsule of this product shall not be less than 0.35mg.