What method does Omon use for ENA inspection? Principle?

Immunoblotting was used. The principle is that firstly, the samples are SDS-PAGE, and the antibodies in them are separated according to the differences of molecular weight and charge, and then transferred to the solid medium-nitrocellulose membrane by in-situ transfer electrophoresis, and the biological activity is maintained, and then the specificity is detected by enzyme immunoassay. I think Omon's autoantibody reagent set is good, but the operation is cumbersome and the price is relatively expensive.