Reshapedantibody is also called CDR implanted antibody, and the variable region of CDRgraftingantibody is the region where antibody recognizes and binds antigen, which directly determines the specificity of antibody. The CDR of mouse monoclonal antibody is transplanted into the variable region of human antibody to replace the CDR of human antibody, so that human antibody can obtain the antigen binding specificity of mouse monoclonal antibody and reduce its heterogeneity. However, although the antigen is mainly in contact with the CDR of the antibody, the FR region is often involved, which affects the spatial configuration of the CDR. Therefore, after switching to human FR region, embedding mouse CDR and V region of human FR may change the configuration of CDR in a single antigen, and the ability to bind to antigen will be reduced or even significantly reduced. At present, the molecular design of the antibody has been able to introduce some key residues from the mouse-derived FR region into the human-derived FR region. If properly configured, its affinity can be comparable to that of the original mouse-derived antibody, but humanized antibody often fails to reach the affinity of the original mouse-derived monoclonal antibody.
Related hot words: antibody chimeric antibody mouse monoclonal antibody affinity specific variable region
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