2. Turn on the fan for 5 ~ 10 minutes, and then conduct the experimental operation after the air in the cabinet is purified and the airflow is stable. Slowly put your arms into the safety cabinet and stand still for at least 1 minute, and then operate after the air flow in the cabinet is stable.
3. There is nothing irrelevant to this experiment in the biosafety cabinet. The articles in the cabinet should be basically separated from the clean area, semi-polluted area and polluted area, and the articles are easy to access during operation, and there is no intersection among the three areas. Keep things in the back as far as possible, but don't block the vent, so as not to interfere with the normal flow of air.
4. The operation should be carried out from the clean area to the polluted area to avoid cross-contamination. In order to prevent possible splashing droplets, a towel or gauze soaked with disinfectant can be spread on the countertop, but the grille of the safety cabinet cannot be covered.
5. When operating in the cabinet, it is forbidden to use open flames such as alcohol lamps, so as to avoid the heat generated to generate airflow and interfere with the stability of airflow in the cabinet; An open flame may damage the HEPA filter.
6. When working, try to reduce the number of people walking behind, and quickly open and close the door to prevent the airflow instability in the safety cabinet.
7, in the experimental operation, don't open the glass window, should ensure that the operator's face is above the work window. When operating in the cabinet, the action should be gentle and slow, so as not to affect the airflow in the cabinet.
8. Safety cabinets should be tested and maintained regularly to ensure their normal work. Once the safety cabinet is found to be abnormal, it should stop working immediately, take corresponding treatment measures and notify relevant personnel.
9. After the work is completed, close the glass window, keep the fan running for 10 ~ 15 minutes, and turn on the ultraviolet lamp for 30 minutes.
10, the safety cabinet should be cleaned and disinfected regularly. After the work is completed, the pollutants on the table in the cabinet can be wiped with 2% 84 disinfectant and disinfected with ultraviolet lamp. The outer surface of the cabinet should be wiped with 1% 84 disinfectant every day.
1 1. Articles used in the cabinet should be taken out after disinfection to prevent pathogenic microorganisms from being brought out to pollute the environment.